Advances in Consumer Research
Issue 3 : 125-148
Original Article
Stability Indicating Analytical Method Development And Validation For The Determination Of Favipiravir By Rp-Hplc
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1
Associate Professor, Department of Pharmaceutical Analysis, VJ’s College of Pharmacy, Rajamahendravaram, Andhra Pradesh
2
Department of Pharmaceutical Analysis, VJ’s College of Pharmacy, Rajamahendravaram, Andhra Pradesh Student.
3
Student, Department of Pharmaceutical Analysis, VJ’s College of Pharmacy, Rajamahendravaram, Andhra Pradesh.
Abstract

A simple, Precised, Accurate method was developed for the determination of Favipiravir  by RP-HPLC technique. Chromatographic conditions used are stationary phase Agilent c18 150 x 4.6 mm, 5m. Mobile phase 0.1% OPA buffer: Acetonitrile in the ratio of 50:50 and flow rate was maintained at 1ml/min, detection wave length was 230 nm, column temperature was set to 30oC and diluent was Acetonitrile: Water (50:50), Conditions were finalized as optimized method. System suitability parameters were studied by injecting the standard five times and results were well under the acceptance criteria. Linearity study was carried out between 25% to150 % levels, R2 value was found to be as 0.999. Precision was found to be 0.7% for repeatability and 1.2% for intermediate precision. LOD and LOQ are 1.140µg/ml and 4.424µg/ml respectively. By using above method assay of marketed formulation was carried out 100.37% was present. The above method can be used in routine quality control analysis.

 

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