The Tree of Life is used in Persian art as a symbol to depict the concepts of continuity, spiritual development and relationship of natural world and the divine world.  This study will discuss the design of the Tree of Life as used in Carpets of Kashan, Tabriz and Herat, which are three major centres of the Persian carpet weaving. The iconography and regional variation of these carpets and the study will be done.  It will also discuss cultural, religious as well as historical reasons that have shaped its representations. The current studies the impact of regional identity on the visual language of the motif, using a systematic study of compositional structure, methods and strengths inherently connected to the style, chromatic pattern and technical qualities. Kashan carpets are often designed in a organized and vertical theme, and this design focuses on highlighting spirituality and design harmony. In contrast, Tabriz carpets were marked by a high degree of naturalism, abundant detailing and a more wide artistic view, which was the evidence of numerous cultural contacts in the region. In addition, Herat examples show combination of geometrical control with flowing vegetable models, and hence depict the historical contribution of the region to art in terms of ethnic exchange. These differences have been emphasized by the study the varied symbolic interpretations of the Tree of Life and in the larger framework of the Persian cultural values in as far as creation, growth, protection, and cosmic harmony are concerned. The findings help to understand better the iconography of Persian carpets and underline the role of the craft in the region in developing the visual and symbolic language of traditional textile work.